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Fig. 1. Immunolocalization of CP4 EPSPS in developing corn anthers at the microspore mother cell stage of development. Panel A: Toluidine Blue O stain of a transverse section through a developing anther showing one microsporangium. Panel B: Control section treated with pre-immune antiserum as primary antibody. Panel C: Section of a P-e35S/CP4 EPSPS event treated with anti-CP4 EPSPS polyclonal antiserum as primary antibody. Panel D: Section of P-Ract1/CP4 EPSPS event treated with anti-CP4 EPSPS polyclonal antiserum as primary antibody. Positive detection is indicated by development of dark punctate reaction product. M: microspore mother cell. T: tapetum cell layer. Size bar = 20 µm.
