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Fig. 4. Southern blot analysis of T1 transgenic rice plants. Lane: P, 5 ng plasmid pYP1203E DNA cut by BglII at a single site was loaded as a positive control; C, a nontransformed control plant; X-1-1, X-2-9, Q-1-5, Q-2-1, H-1-1, and Y-1-1, representative T1 transgenic plants from their T0 progenies respectively (Plant genomic DNA digested with BglII. X stands for ‘Xiushui-11’, Q for ‘Qiufeng’, H for ‘Hanfeng’, and Y for ‘Youfeng’). The three polymerase chain reaction products were used as probes and labeled with DIG-Prime (digoxigenin; Roche Applied Science, Penzberg, Germany). The same southern filter was rehybridized with different probes. (A) VHb, Vitreoscilla hemoglobin gene; (B) tzs, trans-zeatin secretion gene; (C) EPSPS, 5-enolpyruvylshikimate-3-phosphate synthase gene.
