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Fig. 1. Schematic representation of the T-DNA region in the pYP1203E plasmid. 5'NOS, 5'-untranslated promoter region of the Agrobacterium tumefaciens nopaline synthase gene. VHb, homodimeric hemoglobin gene; 3'rbcs, 3'-untranslated terminator region of the Arabidopsis rbcs gene; SAR68, chromosome matrix-attachment region (MAR) of tobacco; 5'STS14, the pistil-specific promoter derived from potato; tzs, trans-zeatin secretion gene; 3'nos, 3'-untranslated terminator region of the nopaline synthase gene; EPSPS, 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) gene; RBCS, the chloroplast-targeting transit peptide RBCS from Arabidopsis at the 5' region of the EPSPS expression cassette; 5'35S, CaMV35S promoter; gus (intron), ß-glucuronidase gene with an intron; 5'D35S, double CaMV35S promoter; hpt, hygromycin phosphotransferase gene; LB, left border; RB, right border; BglII cut the plasmid DNA at a single site between 5'35S and GUS gene sequence.
