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Fig. 1. Somatic embryogenesis on the abaxial side of immature cotyledons under hygromycin selection following A. tumefaciens strain KYRT1-mediated transformation. A, B, and C, somatic embryos formed singly, in clusters, or as polyembryogenic structures, respectively. Histochemical GUS staining of responding explants are shown in (A) and (B). D, an opaque-type globular somatic embryo. E, a fused-type structure of translucent- and opaque-type SEs indicated by arrows. F, proliferative embryogenic tissues derived from an opaque-type SE.
