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Dep. of Agronomy, Univ. of Kentucky, Lexington, KY 40546-0091
* Corresponding author (nltaylor{at}uky.edu)
Red clover (Trifolium pratense L.) genetic marker stock (L38-1810) (Reg. no. GS-13, PI 633760) was released by the Kentucky Agricultural Experiment Station in 2003. It was discovered in 1995 in an inbred multiple-cotyledon genetic stock (L38-1485) in which a single plant expressed shortened internodes but normal-sized leaflets (Taylor and Mundell, 1999). Some leaves have only a single leaflet and others have three leaflets with an elongated central leaflet stalk. Short internode plants usually present a rosetted appearance but flower sparingly under long daylengths.
The progeny of short internode plants crossed with normal plants in 1996 were all normal, suggesting the character was inherited in a recessive manner. Selfs and intercrosses of short internode plants bred true. In further crosses made in 1999, intercrossed heterozygous normal plants produced progeny segregating a close fit to a 3:1 ratio (124:42). Twenty-four test crosses of heterozygous normal produced progeny segregating to a reasonable fit to a 1:1 ratio (145:120). Based on these data, we conclude that a single recessive gene is responsible for the short-internode condition. We suggest that the symbol (ab) be assigned for this gene. Accordingly, the normal condition would be AbAb or Abab, and short internode, abab. This gene symbol has not been used for a genetic marker in red clover (Quesenberry et al., 1991).
In the summer of 2003, short internode plants were hand crossed in a greenhouse to produce seed. Up to 10 seeds of this genetic marker stock may be obtained upon written request and agreement to return increased seed to the corresponding author.
NOTES
The investigation reported in this paper (97-06-6) was in connection with a project of the Kentucky Agric. Exp. Stn., Lexington and is published with approval of the Director. Registration by CSSA.
Accepted for publication December 31, 2003.
REFERENCES
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