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Fig. 5. DNA sequence alignment of partial catalytic subunit genes amplified from genomic DNA of CDC Teal, TealIMI 10A, TealIMI 11A, TealIMI 15A, and BW755. Only relevant sequence is presented with complete sequences available on GenBank (accession numbers presented on figure). Imi1 and Imi2 and wild-type sequences (1816 bp) were PCR cloned by means of primers AHAS17FWD and AHAS30REV. Nucleotide sequences of Imi3 and imi3 (617 bp) were PCR cloned by means of primers AHAS21FWD and AHAS26REV. The ruler represents the amino acid residue no. of AHAS from O. sativa. Single nucleotide polymorphisms between the three partial wheat catalytic subunit genes are indicated by a box.
