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Fig. 4. Southern blot hybridization of total DNA from T₁ plants digested with EcoRI, which cuts only once within the plasmid, outside of any gene cassette, and probed with a 633-bp amplicon of the nptII gene. Plants numbered 21 through 24 survived 400 mg L^–1 kanamycin, and were the parents for the T₂ plants.

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