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a Dep. of Agronomy, Purdue Univ., West Lafayette, IN 47907
b Dep. of Crop Sci., North Carolina State Univ., Raleigh, NC 27695
* Corresponding author (cbigelow{at}purdue.edu).
| ABSTRACT |
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Abbreviations: AWHC, available water holding capacity IA, inorganic amendment Ksat, saturated hydraulic conductivity OM, organic matter SP, sphagnum peat moss SWP, soil water pressure WHC, water holding capacity
| INTRODUCTION |
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A number of inorganic materials, including porous ceramics, diatomaceous earth, and zeolites, are currently marketed as alternatives to SP for construction of sand-based rootzones. These products are generally stable, very porous, and are designed to increase microporosity and, thereby, water retention. Most are sized comparable with sand (
2 mm) to maintain high percolation rates. Many have been evaluated with mixed success (Waddington et al., 1974; Schmidt, 1980; Ferguson et al., 1986; Nus and Brauen, 1991; Kussow, 1996; McCoy and Stehouwer, 1998; Miller, 2000; Waltz et al., 2003; Wehtje et al., 2003). The major criticism of IAs is that much of the internally held water is not plant-available (Davis et al., 1970; Waddington et al., 1974). By contrast, Van Bavel et al. (1978) reported that fritted clay was an excellent medium for plant growth, providing good aeration and containing 0.31 cm3 cm3 of available water, much of it held internally. Unfortunately, few of the aforementioned studies contain results that directly compare IAs to an appropriate SP control, which makes data interpretation difficult.
Therefore, the overall objective of this study was to evaluate several commercially available IAs for potential use in newly constructed putting green rootzones. Specific objectives were (i) to evaluate selected physical properties of several currently marketed IAs alone and when mixed with fine, medium, and coarse sand; and (ii) to compare physical properties of inorganically amended sands with sand amended with a traditional organic amendment, SP.
| MATERIALS AND METHODS |
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Total porosity was calculated with measured bulk density and particle density as determined by the pycnometer method (Flint and Flint, 2002a). Macroporosity (air-filled) was calculated by subtracting the water content at 0.004 MPa from total porosity. Microporosity (capillary water) was defined as the amount of pores retaining water at 0.004 MPa (USGA, 1993). Available water holding capacity (AWHC) of each material was calculated as the difference between water retained at 0.004 MPa and 0.05 MPa.
Additional physical properties were determined in situ with 30-cm-deep rootzone mixtures, equivalent to the compacted depth of most sand-based putting green rootzones. Columns (7.6-cm i.d. by 35 cm tall, with a wall thickness of 0.42 cm) were constructed from acrylic tubing and equipped with access measurement ports (1.8-cm diam.) located at 2-cm intervals in a spiral arrangement down the sides of each column corresponding to depth intervals from 2 to 26 cm below the surface of the media. During use, each measurement port was covered by a rubber stopper. Additionally, stainless steel mesh was embedded into the base of each column and before packing the columns, the steel mesh base was fitted with a single sheet of porous glass wool to retain the sand mixtures in the columns.
Conventional laboratory methods for determining the physical properties of potential sand-based rootzone media require samples to be compacting from the top using a weighted hammer apparatus (USGA, 1993). These tests are normally conducted with small steel cylinders (5-cm diam. by 7.610 cm tall), not the full 30-cm rootzone depth. Because of the unique in situ column approach (7.6-cm diam. by 30 cm tall) used to determine moisture content with depth, a preliminary study was conducted to determine the most effective packing method which would not disturb the sidewall measurement ports. Sand and amended sands were installed in smaller, more traditional columns and compacted to determine the mass of sand or sand + amendment required to produce a compacted 30-cm-deep rootzone. On the basis of these measurements, air-dry sand or sand + amendment were preweighed, mixed, and installed into the larger columns by slowly pouring in one continuous step. This process minimized layering and maintained the integrity of the measurement ports. The media was further compacted by hand through repeated tapping on a hard surface until the rootzone mixtures were exactly 30 cm deep.
In addition to the 10 and 20% rates, each amendment was also evaluated at two incorporation depths: throughout the entire 30-cm depth, (referred to as throughout) and incorporated only in the top one half of the 30-cm rootzone (hereafter called top half) with three replications of each treatment for all measurements.
When the amendment mixture was placed only in the top half of the rootzone, it was subtended by 15 cm of unamended sand of the same size class. Packed columns were incrementally saturated with tap water from the bottom up until ponding at the rootzone surface was observed. After 24 h, Ksat was determined by the constant head method (Klute and Dirksen, 1986), with results adjusted to 20°C.
After Ksat was measured, each column was loosely capped to prevent evaporation, placed on a screen drying rack, and allowed to drain for 24 h. Horizontal cores (1-cm diam. x 7.5 cm tall) of the media were then sampled at each access port and oven dried for 24 h at 105°C to determine gravimetric water content.
Bioassay for Available Water
The amount of available water held by some of the amendments, as obtained with standard desorption techniques (see above), seemed surprisingly low and were in direct conflict with results documenting considerable available water in other calcined clays (Van Bavel et al., 1978). Consequently, a bioassay was designed to estimate plant available water in fine sand and calcined clay, with a combination of tensiometers for soil water potentials <100 kPa, and leaf water potential as a lagging indicator of more negative soil water potential. Plastic pots (15.2-cm diam. by 11.1 cm deep) were fitted with two 1-cm diam. tensiometers (Soil Moisture Systems, Las Cruses, NM) located on each side of the pot, 5 cm above the bottom. A layer of cheesecloth was placed in the bottom of the pot which was then filled to a depth of 10 cm with the fine sand or the calcined clay. Perennial ryegrass (Lolium perenne L. Competitor) was seeded at 250 kg ha1 and grown in the greenhouse (36/21°C day/night) for 10 wk, during which the plants were well watered and fertilized with 14 kg N ha1 wk1 from a soluble (20-20-20 N-P-K) fertilizer. The grass was unmowed during the study to maximize transpiration.
With the grass well established, a 4-d drought stress period was imposed. Soil water content was determined gravimetrically each day (corrected for plant biomass), and soil matric potential was determined directly with the tensiometers (low tensions) and indirectly by measuring leaf water potential (higher tensions). Leaf water potential was measured with a hydraulic press (J-14 Leaf Press, Decagon Devices, Pullman, WA) technique (White et al., 1992). Briefly, three representative leaves were removed at each sampling period, the lamina segments (approximate length of 5 cm) were placed on filter paper within the press and pressure applied until sap was expressed. Six replications of each rootzone amendment were used in a completely random experimental design and plants were moved every other day to offset effects of possible environmental gradients within the greenhouse.
All data was subjected to ANOVA with the Statistical Analysis System (SAS Institute, 1985). Separation of significantly different treatment means was accomplished by preplanned orthogonal contrasts (Steel et al., 1997). Means were separated with Fisher's protected LSD if the ANOVA F test indicated that source effects were significant. Amended sand rootzone mixtures within each sand class were compared with the unamended sand control by Dunnett's test (Steel et al., 1997). The laboratory experiments were conducted with a completely random factorial design. The pore size distribution and water retention data were analyzed as a two-factor study (sand size and incorporation rate) and the in situ study as a three-factor study (sand size, incorporation rate, and incorporation depth).
| RESULTS AND DISCUSSION |
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0.15 cm3 cm3 but <0.25 cm3 cm3 volumetric water (Bingaman and Kohnke, 1970; USGA, 1993), suggesting that both the medium and coarse sand might be difficult to manage without amending to improve water retention. However, it must also be considered that the sands used in this study were screened to a high uniformity not available in practice. Consequently, the medium sand discussed here, as an example, might not be easily compared with a predominantly medium sand from a commercial source.
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The moisture characteristic of the substrate is extremely important for successful putting green rootzones. Consequently, data on moisture release from IAs should help in selecting appropriate amendments for sand-based rootzones. For example, if an amendment releases most of its water at a relatively low tension and retains little at a moderate tension, it may contribute little benefit to a coarse-textured sand. Conversely, if an amendment releases little water at low tensions and retains significant quantities at higher tensions, making it unavailable to the turf, it may be equally unsuitable. In the present study, all amendments except fine sand released 0.28 to 0.36 cm3 cm3 water between saturation and 0.002 MPa. In constructed rootzones deeper than 20 cm, this water would be lost through gravitational drainage and thus unavailable for plant use. In contrast, fine sand released only 0.004 cm3 cm3 water at this low tension. Thus, the fine sand retains a substantial amount of water that may be available for plant growth.
To characterize further the moisture release properties of the amendments and three sands, water retention data were collected for a range of soil water pressures (SWPs). Each sand and amendment generally had a characteristic tension at which much of the water was released (Fig. 1) . For the sands, this critical SWP is related to the particle size, with coarse sand abruptly releasing water between 0.001 and 0.002, medium sand between 0.001 and 0.004, and fine sand between 0.002 and 0.01 MPa. Compared with the sands, the IAs and SP contained significantly more water at saturation, >0.55 cm3 cm3, and released this water more gradually with decreasing SWP up to 0.006 MPa. At SWP < 0.006 MPa, water release from the IAs leveled off and remained relatively constant to 1.5 MPa SWP. Peat moss had the most gradual release for any of the rootzone components.
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Amendments had little effect on macroporosity in all three of the sands, but increased capillary porosity in the medium and coarse sand classes (Table 3). Amendments also increased the moisture held at 0.05 MPa. However, increased capillary porosity did not translate into increased AWHC. Inorganic amendments either had no effect on AWHC (medium and coarse sands) or, as in the fine sand, actually decreased AWHC. Not surprisingly, 20% SP increased AWHC in the medium and coarse, but not the fine sand. It should be noted that AWHC was extremely low for both the medium and coarse sands, even with amendment addition. This is probably because of the very high uniformity of the sands used, and suggests that some highly sorted sands might actually have too narrow a particle size distribution for adequate moisture-holding capacity. Thus, these sands would benefit from a small quantity of finer-sized particles.
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Bulk Density
Amendments decreased bulk density (Table 3) for all three sand classes, with SP having the greatest effect. Similar results were observed by Juncker and Madison (1967), Waddington et al. (1974), and Waltz et al. (2003). Bulk density alone, however, is not considered to be an adequate indicator of a successful rootzone mixture (USGA, 1993).
Saturated Hydraulic Conductivity
The Ksat values were very high for all three sands, and increased with coarseness (89, 211, and 505 cm h1 for the fine, medium, and coarse sand, respectively, data not shown). Amendments decreased Ksat 13 to 50% in the medium sand, with the reduction directly related to the geometric mean diameter of the incorporated amendment. There was less effect of amendment on fine sand, and essentially no effect on the coarse sand. Mean Ksat values for each amendment across all three sand classes ranked in the following order: vitrified clay = zeolite
unamended sand
diatomaceous earth
calcined clay > SP. Amending only the top 15 cm had less impact on Ksat than incorporation throughout the entire 30-cm rootzone; the 20% amendment rate decreased conductivity more than the 10% rate (data not shown).
These Ksat values are much higher than the USGA guidelines of 15 to 30 cm h1 (USGA, 1993), most likely because of our use of highly uniform sands. This is consistent with results of Bingaman and Kohnke (1970), who reported similar high Ksat values for several well-graded fine and medium sands.
Water Retention and Availability of Simulated Rootzones
Soil moisture profiles (Fig. 2)
varied considerably, depending on sand size. All three sands were close to saturation (
0.45 cm3 cm3) at the bottom of the 30-cm sand column, where the gravitational head was zero. There was a curvilinear decrease in soil moisture with height, with the greatest change occurring in the coarse sand and the least in the fine sand. At the top of the column, soil moisture had declined to 0.04, 0.17, 0.36 cm3 cm3 for the coarse, medium, and fine sand, respectively. The coarse sand held very little water in the top 15 cm of the 30-cm rootzone, whereas the fine sand remained relatively wet throughout the entire rootzone. If used unamended, both sands would present management challenges, with the coarse sand being too droughty and the fine sand lacking adequate aeration, except perhaps in the upper 5 cm of the soil profile. The medium sand appears best suited for shallow (<30 cm) rootzones, based on the balance of water-filled and air-filled pores throughout much of the rootzone.
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0.15 cm3 cm3 is necessary for seedling survival and establishment (Bingaman and Kohnke, 1970). Field studies with sand-based rootzones showed that volumetric water retention <0.09 cm3 cm3 resulted in poor turfgrass establishment and required careful maintenance (Carlson et al., 1998). On the basis of these results, moisture content at the surface of both the medium and fine sands, with or without amendments, would be considered adequate for successful turf establishment. Amendments also increased water retention at the surface of the coarse sand. However, with the exception of sand plus 20% SP, none of the coarse sand mixtures retained sufficient water at the surface (>0.15 cm3 cm3) to assure success. Available soil moisture is more important than total water content, at least for turfgrass performance. We calculated the amount of available water in the top 15 cm of the 30-cm rootzone for each sand mixture. This depth was chosen as it contains most of the root system. Available water was determined by subtracting the 0.05 MPa value (unavailable) from the volumetric water content measured at each sampling depth, and averaging the results for the entire 15 cm (data not shown). Amendments tended to increase total water retention but had little or no effect on available water. Some of the IAs actually decreased AWHC in the fine and medium sands. Peat was the only amendment that significantly increased available water, and then only in the coarse sand.
Bioassay for Plant Available Water
As discussed above, IAs hold considerable moisture, but much of it appears to be unavailable, at least when determined with standard laboratory techniques. This conflicts with the results of Van Bavel et al. (1978) and McCoy and Stehouwer (1998), showing that much of the water contained by some porous IAs is released at tensions consistent with plant availability. One possible explanation for this disparity considers that the pore structure of the IAs might be discontinuous, and that some pores would thus be isolated and disconnected from the tension source of a pressure plate. As such, some fraction of the pores might not drain, even at high tensions. This would result in exaggerated values for unavailable moisture, and reduce the calculated values for available water.
In an attempt to reconcile this disparity, we designed a bioassay to determine available water with evapotranspiration (and root absorption) as the driving force for water extraction. We hypothesized that the extensive turfgrass root system, including its profusion of root hairs, would be able to contact and access water held in isolated and disconnected pores of the IA. Calcined clay was chosen for study, since it is highly porous and as determined with the pressure plate, retained large amounts (>0.25 cm3 cm3) of unavailable water. Perennial ryegrass was selected as a fast-growing species that develops a very dense root system.
The moisture release curves for the sand and Profile were similar in form to those generated with standard methods of physical analysis (Fig. 5) . There was good agreement between the two methods for both the fine sand and calcined clay, particularly in the tension range at which most of the water was released. However, there was an important divergence between the two methods for calcined clay at SWP > approximately 100 kPa, with the bioassay indicating greater water removal (>0.10 cm3 cm3) than the pressure plate method. This result indicates that the ryegrass plants were able to access and extract much of the capillary water and implies that calcined clay, and perhaps the other porous IAs, hold considerably more available water than standard pressure plate methods might suggest.
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| CONCLUSIONS |
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| ACKNOWLEDGMENTS |
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| REFERENCES |
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