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Fig. 4. Southern blotting analyses of oilseed rape cuphea-TE transgenic parental line TL6 and DH2 seedlings originating from eight DH1 plants by probing with the cuphea-TE transgene probe (a) followed by re-probing with the nptII gene probe (b) and schematic representation of the position of the probes in the T-DNA construct, not drawn to scale (c). The estimated sizes of the bands are shown in kilobase pair (kb). Lanes 1 to 8 represent the eight DH1 plants that were developed from crosses of the cuphea-TE transgenic parental line TL6 with nontransgenic plants and showed cuphea-TE expression by enhanced levels of palmitic acid (18.7–35.5% palmitic acid) in DH2 seed oil; P, a plasmid carrying the cuphea-TE transgene; CK, nontransgenic control plants. LB, left T-DNA border; RB, right T-DNA border; 35S, CaMV 35S promoter; tml, tml 3' region; nptII, neomycin phosphotransferase gene; napin 5', a seed specific promoter of the napin gene from B. rapa; napin 3', 3' termination fragment of the napin gene; cuphea-TE, the cuphea-TE transgene. Hatched bars represent the probes for the nptII gene and the cuphea-TE transgenes. The NsiI site is approximately 1.9 kb from RB and 5.9 kb from LB (Jones et al.., 1995; Kridl et al.., 1991; McBride and Summerfelt, 1990).
