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REGISTRATIONS OF GERMPLASMS

Registration of KS00WGRC44 Leaf Rust-Resistant Hard Red Winter Wheat Germplasm

^a USDA-ARS and Dep. of Agronomy, Kansas State Univ., Manhattan, KS 66506-5501
^b Dep. of Agronomy, Kansas State Univ., Manhattan, KS 66506-5501
^c Land Institute, Salina, KS

^* Corresponding author (gbg{at}ksu.edu)

KS00WGRC44 (Reg. no. GP-744, PI 632343) is a hard red winter wheat (Triticum aestivum L.) with resistance to leaf rust [caused by Puccinia triticina Eriks. = P. recondita Roberge ex Desmaz. f. sp. tritici (Eriks. & E. Henn.) D.M. Henderson] developed cooperatively by the ARS, USDA, the Kansas Agricultural Experiment Station, and the Wheat Genetics Resource Center. It was released as germplasm August 2000.

KS00WGRC44 is a BC₂ F₆–derived line with the pedigree ‘TAM 107’*3/TA 1715. TAM 107 is a hard red winter wheat cultivar (Porter et al., 1987), and TA 1715 is a leaf rust resistant accession of Aegilops tauschii Coss. collected in Iran. The F₁ hybrids between TAM 107 and TA 1715 were produced in the greenhouse in 1995 using TAM 107 as the female parent. F₁ embryos were rescued on artificial media (Gill and Raupp, 1987) and F₁ plants were backcrossed as females to TAM 107. The BC₁ F₁ plants were backcrossed as females to TAM 107 and the BC₂ F₁ plants were grown in the greenhouse to produce the BC₂ F₂ generation. The pedigree breeding method was used to develop the BC₂ F₆–derived line with selection in the field and greenhouse for leaf rust resistance and recurrent parent plant type.

Seedlings of KS00WGRC44 exhibited a low infection type of small necrotic uredinia [IT = 1 or lower on a scale of 0 to 4 (Roelfs et al., 1992)] when inoculated with 15 races of leaf rust, CBB-10,18, CDB-10, KDB-10, LBB-10,18, MCR-10, MCD-10, MBG-10,18, MBR-10, MFB-10, PBB-10, PBJ-10, PNM-10,18, TBD-10, TCR-10, and TFG-10 (Long and Kolmer, 1989). High infection types of 3 to 4 (moderate to large uredinia, lacking chlorosis or necrosis) were observed on seedlings of TAM 107 with all the races of leaf rust tested. Adult plants of KS00WGRC44 displayed a hypersensitive fleck (IT = ;) level of resistance when exposed to moderate to heavy leaf rust inoculum levels in the field at Manhattan and Hutchinson, KS, in 1999, 2000, and 2001 and under heavy inoculum pressure at Uvalde and Beaumont, TX, in 2000.

Leaf rust resistance in KS00WGRC44 is due to a single dominant gene from TA 1715. Differences in infection type of seedlings when inoculated with diverse isolates of P. triticina indicate that the gene in KS00WGRC44 is different from the Ae. tauschii-derived genes Lr32, Lr39, Lr41, and Lr42. KS00WGRC44 had a lower infection type of 1 (flecks with small necrotic uredinia) when inoculated with leaf rust race PNM-10,18 at the seedling stage than that observed on the lines TA 4186 (Lr39; IT = 4) and KS90WGRC10 (Lr41; IT = 3C, moderate size uredinia with chlorosis). When seedlings of KS00WGRC44 and RL5713 (Lr32) were inoculated with the TFG-10 race of leaf rust, ITs 0 (no visible infection) and 2+ (small to moderate size uredinia with chlorosis) were observed, respectively. When inoculated with race KDB-10, seedlings of KS91WGRC11 (Lr42) had an IT 2C (small uredinia with chlorosis) while no sporulation was observed on seedlings of KS00WGRC44 (IT = ;). Absence of the 1.36-kb fragment amplified by the primer pair KSUD14, which corresponds to a portion of the cloned Lr21 gene, indicates that the gene in KS00WGRC44 is different from Lr21 (Huang et al., 2003).

When evaluated in replicated field plots at Manhattan and Hutchinson, KS, in the 2000 and 2001 growing seasons, the heading date of KS00WGRC44 was within one day of the recurrent parent. The mean yield and test weight of KS00WGRC44 was not significantly different than that of TAM 107. However, KS00WGRC44 was significantly taller than TAM 107 (110 vs. 102 cm, respectively).

Small quantities (2 g) of seed of KS00WGRC44 are available upon written request. Appropriate recognition of source should be given when this germplasm contributes to research or development of a new breeding line or cultivar. Seed stocks are maintained by the Wheat Genetics Resource Center, Dep. of Plant Pathology, Throckmorton Plant Sciences Center, Kansas State University, Manhattan, KS 66506-5502.

NOTES

Joint investigation by the USDA-ARS and the Kansas Agricultural Experiment Station. Contribution number 03-123-J. Registration by CSSA.

Accepted for publication August 31, 2003.

REFERENCES

• Gill, B.S., and W.J. Raupp. 1987. Direct genetic transfers from Aegilops squarrosa L. to hexaploid wheat. Crop Sci. 27:445–450.[Abstract/Free Full Text]
• Huang, L., S.A. Brooks, W. Li, J.P. Fellers, H.N. Trick, and B.S. Gill. 2003. Map-based cloning of leaf rust resistance gene Lr21 from the large and polyploid genome of bread wheat. Genetics 164:655–664.[Abstract/Free Full Text]
• Long, D.L., and J.A. Kolmer. 1989. A North American system of nomenclature for Puccinia recondita f. sp. tritici. Phytopathology 79:525–529.[ISI]
• Porter, K.B., W.D. Worrall, J.H. Gardenhire, E.C. Gilmore, M.E. McDaniel, and N.A. Tuleen. 1987. Registration of TAM 107 wheat. Crop Sci. 27:818.[Free Full Text]
• Roelfs, A.P., R.P. Singh, and E.E. Saari. 1992. Rust diseases of wheat: Concepts and methods of disease management. International Maize and Wheat Improvement Center (CIMMYT), Mexico.

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