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Fig. 5. Southern blot analysis on T2 individuals derived from marker-free T1 seed of line 420-5. Upper panel, 
6 desaturase probe. Lower panel, bar gene probe. Lanes 1-6, T2 individuals from marker-free population of line 420-5. Lane 7, wild-type (WT) soybean. Lane 8, 50 pg pPTN331 positive control. DNAs digested with EcoRI. Numbers above the column refer to the percentage of GLA in the T2 seed sample. HS indicates all individuals were scored herbicide sensitive.
