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Fig. 4. (A) Southern blot analysis on herbicide-sensitive/modified fatty-acid profile (HS/GLA/STA) T1 individuals derived from lines 419-2 and 420-5. Top panel, 
6 desaturase probe. Lower panel, bar gene probe. Lanes 1 to 4, T1 individuals from line 419-2. Lanes 5 to 8, T1 individual from line 420-5. Lane 9, wild-type (WT) control. Lane 10, 50 pg of pPTN331 positive control. DNAs digested with Sst I. Numbers above each column indicate percentage of GLA in the fatty-acid analysis. HT and HS refer to herbicide tolerant and herbicide sensitive, respectively. (B) Southern blot analysis on HS/GLA/STA T1 individuals derived from lines 411-5, 414-5, and 418-6. Figure description as in (A). Lanes 1 and 2, T1 individuals from line 411-5. Lanes 3 to 6, T1 individuals from line 414-5. Lanes 7 to 10, T1 individuals from line 418-6. Lane 11, wild-type (WT) control. Lane 12, 50 pg of pPTN331 positive control. (C) Southern blot analysis on GLA/STA T1 individuals derived from lines 414-2 and 414-3. Figure description as in (A). Lanes 1 to 4, T1 individuals from line 414-2. Lane 5, T1 individual from line 414-3. Lane 6, wild-type (WT) control. Lane 2, 50 pg of pPTN331 positive control.
