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REGISTRATIONS OF GERMPLASM

Registration of Two Germplasms of Rye with Introgressions of the Glu-D1 Locus from Chromosome 1D of Bread Wheat

^a Dep. of Botany and Plant Sciences, University of California, Riverside CA 92521-0124
^b Research Center for Cultivar Testing, 63-022 Slupia Wielka, Poland

^* Corresponding author (ajoel{at}ucrac1.ucr.edu)

Two rye (Secale cereale L.) germplasms were developed by cytogenetic chromosome engineering to introduce the Glu-D1d locus from chromosome 1D of bread wheat (Triticum aestivum L.)(UCRR1-2001, Reg. no. GP-3, PI 628642; UCRR2-2001, Reg. no. GP-4, PI 628643). Locus Glu-D1d encodes the high molecular weight (HMW) glutenin subunits 5+10 that are considered critical for good breadmaking quality in wheat (Payne, 1987). Both germplasms carry fragments of chromosome 1D with the Glu-D1d locus inserted into chromosome 1R, where they replace rye locus Sec-2. Locus Sec-2 encodes high molecular weight secalins.

The original cytogenetic manipulations were performed in hexaploid triticale (xTriticosecale Wittmack) ‘Rhino’ where two translocations of Glu-D1d to 1R were produced by homeologous recombination in plants with chromosome constitution 20" + 1RS.1DL' + 1R' (Lukaszewski and Curtis, 1992). Recombination was induced by the removal of the Ph1 locus by a substitution 5D(5B). Translocated chromosome 1R.1D₅₊₁₀-1 was transferred to diploid rye by backcrosses and electrophoretic screening (Lukaszewski et al., 2000). During the transfer, at least two crossover events must have taken place that reduced the length of the introgressed segment of 1D. UCRR1-2001, also known as H510, appears to carry a range of 1DL segments of various lengths. UCRR2-2001, also known as HMA510, was produced following a selection for high male transmission rate of the translocated 1R chromosome and appears to carry small (er) introgressions of 1D. Both germplasms are outcrossing populations of winter rye with no indications of inbreeding depression. The pedigree of UCRR1-2001 is Rhino 1R.1D₅₊₁₀-1/Snoopy//5*Dankowskie Zlote; the pedigree of UCRR2-2001 is Rhino 1R.1D₅₊₁₀-1/Snoopy//5*Dankowskie Zlote/3/3*Motto/4/2*Amilo.

In all preliminary tests, introduction of Glu-D1 into rye improved the parameters of breadmaking quality. The SDS-sedimentation value increased by 33 to 44% and the baking scores in test bakes performed by means of wheat technology were improved by 188 to 257% relative to control population without Glu-D1. The results of bake tests performed by means of rye technology were not affected (Lukaszewski et al., 2000). Introduction of a segment of wheat chromosome 1D into diploid rye reduced 1000-kernel weight in UCRR1-2001 by 15% relative to its control population (Lukaszewski et al., 2000), which reduced yield. This reduction must have been associated with excessive fragment length as it was not detected in UCRR2-2001.

These ryes may be used in wheat–rye blends or as components of new triticale lines. Small quantities of seed for research and breeding purposes can be obtained from the first author.

NOTES

Registration by CSSA.

Accepted for publication April 30, 2003.

REFERENCES

• Lukaszewski, A.J., and C.A. Curtis. 1992. Transfer of the Glu-D1 gene from chromosome 1D of breadwheat to chromosome 1R in hexaploid triticale. Plant Breed. 109:203–210.
• Lukaszewski, A.J., W. Brzezinski, and E. Klockiewicz-Kaminska. 2000. Transfer of the Glu-D1 locus encoding high molecular weight glutenin subunits from breadwheat to diploid rye. Euphytica 115:49–57.
• Payne, P.I. 1987. Genetics of wheat storage proteins and the effect of allelic variation on breadmaking quality. Annu. Rev. Plant Physiol. 38:141–153.[ISI]

This Article Full Text (PDF) Free Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in ISI Web of Science Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Lukaszewski, A.J. Articles by Brzezinski, W. Search for Related Content PubMed Articles by Lukaszewski, A.J. Articles by Brzezinski, W. Agricola Articles by Lukaszewski, A.J. Articles by Brzezinski, W.