Click on image to view larger version.

Fig. 3. OMT activity of 2-wk-old (5-leaf stage) plants: (A) Control plants, (B) A-group T1 transgenic plants. Plants initiated from five seeds resulting from self-pollination of 33 selected primary transgenic (T0) plants were established and grown in the greenhouse. The second youngest leaf from transgenic and control plants were assayed for OMT activity two weeks after planting. The mean of 10 control plants was considered as 100% (0.10 pkat/mg protein). The 33 plant families are labeled under the graph and individuals within a family were designated A1 to A5 sequentially from left to right (not labeled on the Fig.). A bm in the family designation indicates that the T0 parent had a brown midrib phenotype. Independent events are signified by nonconnecting lines below the family designations.