Click on image to view larger version.
Fig. 1. Schematic representation of an antisense sorghum omt vector used for maize transformation. The vector contained the CaMV 35S promoter to drive bar with the nos terminator; and the maize Ubi-1 promoter (plus first intron and first exon) to drive the sorghum omt full-length cDNA (1600 bp), or the 995-bp 5'-fragment, in the antisense orientation with the nos terminator. Restriction sites labeled are; H = HindIII, B = BamHI, and E = EcoRI. Digestion with EcoRI releases a 2.5-kb fragment with the full-length omt or a 1.9-kb fragment with the 995-bp omt vectors.
