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Fig. 1. Polymerase chain reaction amplification of the 446-base-pair (bp) bar gene coding sequence fragment. A. Interspecific hybrids originating from crosses with creeping bentgrass transgenic line 5061. Lane 1, 100-bp marker; lane 2, creeping bentgrass transgenic line 5061; lane 3, colonial bentgrass hybrid; lane 4, velvet bentgrass hybrid; lane 5, dryland bentgrass hybrid; lane 6, redtop bentgrass hybrid; lane 7, nontransgenic colonial bentgrass; lane 8, nontransgenic velvet bentgrass; lane 9, nontransgenic dryland bentgrass; lane 10, nontransgenic redtop bentgrass. B. Interspecific hybrids originating from crosses with creeping bentgrass transgenic line 4475. Lane 1, 100-bp marker; lane 2, creeping bentgrass transgenic line 4475; lane 3, colonial bentgrass hybrid; lane 4, velvet bentgrass hybrid; lane 5, redtop bentgrass hybrid; lane 6, no DNA control. The numbers to the left of the markers indicate the sizes of the marker bands in bp.
