Fig. 2. Silver stained polyacrylamide gel showing polymorphic bands associated with the Yr5 resistance. A: Codominant STS markers amplified with primers Yr5STS-7 (5'-GTACAATTCACCTAGAGT-3') and Yr5STS-8 (5'-GCAAGTTTTCTCCCTATT-3'). The higher band (478 bp) was present in the resistant parent (the Yr5 NIL) and the resistant BC₇:F₃ bulk and the lower band (472 bp) was present in the susceptible BC₇:F₃ bulk and the susceptible parent (AVS for ‘Avocet Susceptible’). B: Codominant STS markers amplified with primers Yr5STS-9 (5'-AAAGAATACTTTAATGAA-3') and Yr5STS-10 (5'-CAAACTTATCAGGATTAC-3'). The higher band (439 bp) was present in the resistant parent (the Yr5 NIL) and the resistant BC₇:F₃ bulk and the lower band (433 bp) was present in the susceptible BC₇:F₃ bulk and the susceptible parent (AVS).

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