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Fig. 4. Amplification of GmFAD3A, GmFAD3B, and GmFAD3C sequences from Williams 82 and A5 genomic DNA. Genomic DNA (50 ng) was subjected to 40 cycles of PCR amplification with primers specific for GmFAD3A, GmFAD3B, or GmFAD3C and products were separated on an agarose gel. W82 lanes contained template DNA from Williams 82, A5 lines contained template DNA from A5, and NEG (negative) lanes contained water in place of template DNA as a control. The predicted product sizes were 149, 139, and 246 bp for GmFAD3A, GmFAD3B, and GmFAD3C, respectively. Molecular weight (MW) lanes contained 100 bp ladder MW markers.
