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Fig. 6. Accumulation of OAS-TL during soybean seed development. (A) Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) analysis of protein profiles of developing soybean seeds. Total seed proteins isolated from six different developmental stages (lanes 1–6) were resolved on a 10% (w/v) SDS-polyacrylamide gel and stained with Coomassie brilliant blue. (B). Western blot analysis. Total protein from developing soybean seeds was resolved by SDS-PAGE, transferred to nitrocellulose, and probed with antibodies raised against the soybean OAS-TL. Note that the antibody specifically recognizes a 34-kDa protein from the soybean seed extracts. The numbers in kilodaltons shown at the sides of the figures represent Bio-Rad (Richmond, CA) protein molecular weight markers (phosphorylase b, 97 400; bovine serum albumin, 66 200; ovalbumin, 45 000; carbonic anhydrase, 31 000; soybean trypsin inhibitor, 21 500; lysozyme, 14 400).
