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Fig. 2. Sequence analysis of the 0.6-kb amplified DNA marker associated with resistance to root knot nematode. In A, the complete nucleotide sequence is shown with the decamer primer binding sites (underlined) and the subsequent CAPS marker primer binding sites (block bold text) indicated within. The four nucleotide sequence (bold italics) comprising an MseI resistriction site polymorphism difference between alleles of the marker is also shown. Translation of a portion of the DNA sequence into encoded protein sequence (B) indicating similarity to the proteins encoded by the PERIANTHA gene from Arabidopsis thaliana (A.t.) and the NIP1 gene from Lycopersicum esculentum (L.e.), both putative transcription factors. An alignment (C) of a portion of the NEM06 marker sequence amplified by the Nem06fwd and Nem06rev primers from both susceptible and resistant sugar beet. The four-base recognition sequence for the restriction enzyme MseI is shown in bold with boxes surrounding the nucleotide difference between the alleles that determines the inability ("G") or ability ("A") for this enzyme to cleave the DNA.
