Click on image to view larger version.
Fig. 2. Strategy for selecting lines (B3)FL3 (Rf3Rf3rf4rf4) and (B3)FL4 (rf3rf3Rf4Rf4). B3Tx398/IS1112C F5:6 individuals were scored for Mmt1, tightly linked to Rf3. Rf3rf3 or Rf3Rf3 individuals were used to pollinate A3Tx398 and resulting sterile plants identified the Rf3Rf3rf4rf4 lines, designated (B3)FL3. Segregants that were mmt1mmt1, and thus rf3rf3, were used to pollinate A3Tx398 to verify the genotype. The line A3Tx398/(B3)FL3 (Rf3rf3rf4rf4) was pollinated with the candidate lines; progeny that yielded only sterile F1s (Rf3rf3rf4rf4) identified rf4rf4 lines, while progeny that segregated 1 fertile:1 sterile identified the rf3rf3Rf4Rf4 lines, designated (B3)FL4.
