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Fig. 9. Sodium dodecylsulfate polyacrylamide gel electrophoresis and immunoblot analysis of buffer-soluble proteins extracted from alfalfa taproots in early December. Twenty-five micrograms of soluble protein were analyzed per lane, and proteins were stained with Coomassie Brilliant Blue R-250 (A). Molecular mass standards (kDa) are listed to the left. Protein gel blots containing 5 µg protein per lane were analyzed using antisera from the 15 and 32 kDa vegetative storage proteins (B). Alfalfa plants were cut in October (C) or uncut (U). Included in the study were the dormant cultivars ‘53Q60’ (Q60) and ‘54H69’ (H69), the nondormant cultivar ‘5939’, and three semidormant germplasms: 96P51PSI (P51), Z57NO2 (NO2), and 96P55PSI (P55). Exp. 2.
