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Fig. 3. Sodium dodecylsulfate polyacrylamide gel electrophoresis and immunoblot analyses of buffer-soluble proteins extracted from alfalfa taproots in late November. (A) Buffer-soluble protein (25 µg per lane) stained with Coomassie Brilliant Blue R-250. Molecular mass standards (kDa) are listed to the left. (B) Protein gel blot containing 5 µg protein per lane was probed using antisera raised to the 15 and 32 kDa vegetative storage proteins. Samples were loaded in lanes according to their respective cutting dates: Lane 1, 7 September; Lane 2, 2 October; Lane 3, 8 October; Lane 4, 15 October; Lane 5, 22 October; Lane 6, 29 October; Lane 7, 6 November; Lane 8, 12 November. Exp. 1.
