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Fig. 1. Examples of DD-PCR results. Amplification of cDNAs, from total RNAs as substrate, was accomplished with (A) primers T11VC and Ap2; (B) primers T11C and AP1; (C) primers T11C and AP1; or (D) primers T11C and Ap7. Amplified cDNAs were from total RNAs. For panels (A) and (B) C2, C4, and C6, RNAs from control leaves of 30-d-old plants watered regularly for 2, 4, and 6 d, respectively; D2, D4, and D6, RNAs from leaves of 30-d-old plants drought-treated for 2, 4, 6 d. For panels (C) and (D) C6 and C9, RNAs from control seedlings held in water for 6 and 9 h; S6 and S9, RNAs from seedlings grown in 250 mM NaCl for 6 and 9 h, respectively. Arrows indicate the differentially expressed cDNA fragments.
