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Fig. 2. Expression of dehydrins in wheat leaves collected after 0 (A), 4 (B), 6 (C), and 12 (D) days of progressive drought stress in seedlings of cultivars Connie (C), Rhode (Rh), TAM105 (T), Hiller (H), Gene (G), Stephens (S), and Rod (R). Nonstress treatments consisted of well-watered plants treated identically to stressed plants in all other aspects. + = positive control (protein extract from Gene wheat subject to drought stress, used only on westerns A and D). Dehydrin proteins were detected using a commercial anti-dehydrin antibody prepared against the consensus dehydrin amino acid sequence EKKGIMDKIKELPG (Close et al., 1993).
