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Fig. 6. Cold acclimation-induced changes in galactinol synthase (msaCIF) transcript (1.4 kB) accumulation in taproots of alfalfa cultivars differing in fall dormancy (FD) and winter hardiness. Norseman (N, FD = 1) is winter hardy, while CUF 101 (C, FD = 9) is not winter hardy. Transcript levels (1.4-kb band) for the parent cultivar (O) and populations from this cultivar selected for greater (L) and less (H) fall dormancy are shown. Roots were sampled at monthly intervals as plants cold acclimated. Equal loading of total RNA per lane (20 µg) was verified by ethidium bromide staining. Experiment 3.
