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Fig. 3. Northern blot analysis of galactinol synthase (msaCIF) transcript (1.4 kB) accumulation in plants of alfalfa. (A) Time course of galactinol synthase transcripts accumulation in crowns of the cold-tolerant cv. Apica during cold acclimation at 2°C and disappearance following transfer at 20°C. (B) Transcript level in leaves and crowns of the cold-sensitive CUF 101 (C) and the cold-tolerant cv. Apica (A) and Rambler (R) that were non-acclimated (N), cold-acclimated two weeks at 2°C (H2) or cold acclimated 2 wk at 2°C followed by 2 wk at -2°C (HF). Assessment of total RNA (10 µg) per lane was made by ethidium bromide staining. Experiment 1.
