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Fig. 1. Amplification of two geminivirus species Cotton leaf curl virus—Pakistan 1 (CLCuV-Pk1) and Cotton leaf curl virus—Pakistan2 (CLCuV-Pk2) by multiplex PCR. The upper band is the PCR product from CLCuV-Pk2 (510 bp) with primers CLCuV-V2091 and PCL2, and the lower band (360 bp) is the PCR product from CLCuV-Pk1 with primers CLCuV-V2091 and CLCuVPk1-C2442. A. Lane M = kb Ladder, Lane 1 = FH-901, Lane 2 = CIM-473, Lane 3 = CIM-482, Lane 4 = CIM-240, Lane 5 = NIAB-98, Lane 6 = MNH-554, Lane 7 = FH-945, Lane 8 = CIM-448, Lane 9 = RH-500, Lane 10 = VH-53, Lane 11 = FH-930, Lane 12 = BH-36, Lane 13 = positive control (radish DNA), Lane M = kb ladder. B. Lane M = kb ladder, Lane 1 = NF-801, Lane 2 = CIM-435, Lane 3 = NIAB-Karishma, Lane 4 = MNH-93, Lane 5 = S-12, Lane 6 = FH-900, Lane 7 = MNH-552, Lane 8 = CIM-443, Lane 9 = BH-118, Lane 10 = CIM-1100, Lane 11 = VH-137, Lane 12 = CP-15/2, Lane 13 = Cedix, Lane 14 = LRA-5166, Lane 15 = positive control (radish DNA), Lane 16 = negative control, M = kb ladder.
