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Fig. 2. CpSSRLP Electropherograms. An automated capillary electrophoretic system showing polymorphic fluorescently labeled fragments amplified with the CpSSRLP CCMP5R/F primer pair. PCR products are visualized as peaks on electropherograms. The x-axis represents the size (bp) of the DNA fragment and the y-axis shows the amount of the amplified products in arbitrary numbers. The red line is the internal size standard included in each electrophoretic run. Callie (1), paternal parent of Tifton 78, Tifton 78 (2), an F1 hybrid (Tifton 44 x Callie), Tifton 44 (3), a maternal parent of Tifton 78 and Coastal (4), a maternal parent of Tifton 44. Local ecotypes, Murphy (5) and Murphy II (6) showing differences in the chloroplast genome. These two ecotypes were included in the figure to emphasize the power of the CpSSRLP technique for differentiating two local ecotypes.
