Fig. 6. Visualization of poly-ß-hydroxybutyrate (PHB) granule accumulation in alfalfa Line 864 transformed with phbABC genes. Leaf tissues stained with Nile Blue A and viewed by confocal microscopy. (A) Untransformed alfalfa; (B) Line 864. Chloroplasts from mature leaves of alfalfa viewed by transmission electron microscopy. (C) Untransformed alfalfa, bar = 1 µm; (D) Transgenic alfalfa with agglomerations of electron-lucent PHB granules (indicated with arrows), bar = 1 µm; (E) Immuno-gold labeling of granules with antibodies against PHB synthase, bar = 0.5 µm.
