Fig. 1. Process of isolated microspore liquid culture for doubled-haploid production, genotype Chris. (a) Mid- to late-uninucleate microspores from the freshly harvested spikes. (b) Embryogenic microspores with fibrillar cytoplasm induced by 2-HNA treatment at high temperature for 65 h. (c) Microspore derived developing embryoids cultured in liquid media for 18 d. (d). Continuous production of mature embryoids upon liquid culture from 21 d on. (e) Germination of embryoids on solid media in Petri dish at 10 d. (f) Thousands of doubled-haploid plants derived from microspores of a single spike.
