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REGISTRATIONS OF GERMPLASMS

Registration of P8901-AP1A2B1B and P8901-AQ1A2B1B Hard Red Spring Wheat Germplasm Lines with Lr21 and Lr34 Leaf Rust Resistance

Semiarid Prairie Agricultural Research Centre, Agriculture and Agri-Food Canada, Box 1030, Swift Current, SK, Canada S9H 3X2

* Corresponding author (knoxr{at}em.agr.ca)

P8901-AP1A2B1B (Reg. no. GP-723, PI 613175) and P8901-AQ1A2B1B (Reg. no. GP-724, PI 613176) hard red spring wheat lines (Triticum aestivum L.), were released in 2000 by Agriculture and Agri-Food Canada (AAFC), Semiarid Prairie Agricultural Research Centre in Swift Current, Saskatchewan. P8901-AP1A2B1B and P8901-AQ1A2B1B derive from the cross ‘Laura’/RL5801//5*Laura. RL5801 [provided by Dr. E. Kerber, Cereal Research Centre (CRC), AAFC] is a hard red spring wheat which carries the gene Lr21 for seedling resistance to the prevalent races of leaf rust (caused by Puccinia triticina Eriks.) found in the Northern Great Plains (Kolmer and Liu, 1997). Laura (DePauw et al., 1988) hard red spring wheat possesses the Lr34 adult plant leaf rust resistance gene (Kolmer, 1994).

Seed of the segregating BC₅F₁, the BC₅F₂, and BC₅F₃ generations, plus Laura and RL5801, were grown in 288 small cavity flats (approximately 2 mL per cavity) watered on demand with 200 µg/mL NPK 20-20-20 nutrient solution and were inoculated at the one and a half leaf stage with race LR145 (provided by Dr. J. Kolmer CRC, AAFC, currently USDA, ARS, St. Paul, MN), which is avirulent on Lr21. Rust inoculation was performed by previously described methods (Stubbs et al., 1986). Plants with resistant reactions (necrosis associated with the pustules and comparable or superior to the resistant parent possessing Lr21) were selected and crossed.

Homozygous resistant BC₅F₄ lines were selected and increased in a winter nursery near Brawley, CA. In 1991, BC₅F₅ and in 1992 BC₅F₆ selected lines were grown near Swift Current, Saskatchewan in four-row plot trials and near Winnipeg, Manitoba in a rust nursery inoculated with leaf rust races (BBB, CBG, FLM, KBB, KBG, KCG, KDB, KFB, MBB, MBG, MDB, MDG, MFB, NBB, TBB, TBD, TBG, TBJ, TDB, TDG, and TFB) found in the Northern Great Plains. P8901-AP1A2B1B and P8901-AQ1A2B1B were selected from among other rust resistant lines on the basis of their overall agronomic performance relative to adapted Canada Western Red Spring wheat cultivars in breeding trials. P8901-AQ1A2B1B has dark chaff and P8901-AP1A2B1B has white chaff. Chaff color is linked to the Lr21 gene (Jones et al., 1990). P8901-AP1A2B1B represents a chaff color Lr21 recombinant.

P8901-AP1A2B1B and P8901-AQ1A2B1B were among 22 lines tested as seedlings to leaf rust race TDT in 1994 and tested in the field in 1995 in a rust nursery near Winnipeg (using races CCD, KBG, MBL, MBQ, MBR, MCR, MDB, MDG, MDL, MDM, MDQ, MDR, MFM, PBM, TBB, TBG, TDB, TDG, TFB, TFG, TFL, TFM, and TFQ). Seedling reactions using races MBR and MFM indicated that Lr21 was present (results provided by J. Kolmer). Seedling and field reactions to leaf rust showed near-complete resistance (mostly necrotic flecks with a few very small sporulating pustules) which was superior to the resistance shown by RL5801 which has the gene Lr21 and to the mesothetic reaction of Lr34 present in the recurrent parent Laura. The superior resistance is consistent with the synergistic effect reported when Lr34 is present with other leaf rust resistance genes (Kolmer, 1997).

P8901-AP1A2B1B and P8901-AQ1A2B1B are similar in height to Laura, which ranges from 73 cm in dry environments to 92 cm in moist environments (DePauw et al., 1988). Unlike Laura both germplasm lines are awnless. Grain yield was measured at Indian Head and Swift Current in 1991 and at Swift Current in 1997. Average yield of P8901-AP1A2B1B was 3673 Kg ha^-1 which was 3.6% lower yielding than Laura (3812 Kg ha^-1) and 23.8% higher yielding than RL5801 (2967 Kg ha^-1). Yield of P8901-AQ1A2B1B averaged 3910 Kg ha^-1 which was 2.6% higher yielding than Laura and 31.8% higher yielding than RL5801. Maturity of both lines were similar to Laura which ranges from 98 d in dry environments to 109 d in moist environments (DePauw et al., 1988). On a one to nine scale where one shows no lodging and nine is completely lodged, P8901-AP1A2B1B and P8901-AQ1A2B1B were similar to Laura which ranges from 1.2 in dry environments to 4.0 in moist environments (DePauw et al., 1988). Like Laura, P8901-AQ1A2B1B was susceptible to loose smut [caused by Ustilago tritici (Pers.) Rostr.] and common bunt [caused by Tilletia caries (DC.) Tul. & C. Tul., T. laevis Kühn in Rabenh.] (results were not obtained for P8901-AP1A2B1B). Small quantities of seed of P8901-AP1A2B1B and P8901-AQ1A2B1B are available from the authors.

NOTES

Registration by CSSA.

Accepted for publication April 30, 2001.

REFERENCES

• Kolmer, J.A., and J.Q. Liu. 1997. Physiologic specialization of Puccinia recondita f.sp. tritici in Canada in 1995. Can. J. Plant Pathol. 19: 166–170.
• DePauw, R.M., T.F. Townley-Smith, T.N. McCaig, and J.M. Clarke. 1988. Laura hard red spring wheat. Can. J. Plant Sci. 68: 203–206.
• Kolmer, J.A. 1994. Genetics of leaf rust resistance in three western Canada spring wheats. Plant Dis. 78:600–602.4.
• Stubbs, R.W., J.M. Prescott, E.E. Saari, and H.J. Dubin. 1986. Cereal Disease Methodology Manual. Centro Internacional de Mejoramiento de Maiz y Trigo (CIMMYT), Mexico.
• Jones, S.S., J. Dvorak, and C.O. Qualset. 1990. Linkage relations of Gli-D1, Rg2, and Lr21 on the short arm of chromosome 1D in wheat. Genome 33:937–940.
• Kolmer, J.A. 1997. Virulence in Puccinia recondita f. sp. tritici isolates from Canada to genes for adult-plant resistance to wheat leaf rust. Plant Dis. 81:267–271.

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