Fig. 2. Differential interference contrast micrographs of cleared buckwheat ovules at anthesis. (A) There is a prominent hypostase (arrow) which is in continuity with the egg sac at the base of the ovule. The synergids, egg, and central cell nucleus are located in the distal part of the ovule near the micropyle (arrowhead indicates outermost cells of nucellus). Bar = 50 µm. (B) The relation between micropyle (arrowhead) and the egg cell complex. In fertile egg sacs, the egg and synergid cells are well defined and in close proximity to the micropyle. Bar = 20 µm. (C) Higher magnification of Fig. 2B showing relationship of the prominent central cell nucleus (arrow) to the synergids (arrowheads). The egg cell tends to lie over or under a synergid such that the central cell nucleus and egg cell are in close proximity to the synergid. In this illustration, the egg cell is not in the plane of focus. Bar = 10 µm. (D) Anthesis ovule with degenerated remains of megagametophyte. Bar = 20 µm.
