Fig. 3. Schematics of the 1067- and 779-bp fragments amplified by SCAR18f/r (a) and SCBC792f/r (b) primer pairs, respectively. BB and bb represent alleles from LA317 and Floradade, respectively. Short arrows at the ends represent the original 10-mer RAPD primers. Longer arrows indicate the locations of 22- or 24-mer SCAR primers. f and r represent forward and reverse SCAR primers, respectively. f1 is the forward SCAR primer used for the dominant SCBC682 marker. Solid triangles indicate the positions of RsaI or HinfI restriction sites.
