Fig. 2. OPAR18 and UBC792 amplified RAPD products, SCAR18f/r and SCBC792f/r amplified SCAR products, and the amplified SCAR products subsequently digested with restriction enzymes. M is a 100-bp DNA ladder. Lanes 1 to 2 (OPAR18) and 7 to 8 (UBC792) are the RAPD products amplified from LA317 (BB) (lanes 1 and 7) and Floradade (bb) (lanes 2 and 8) parents. Lanes 3 to 4 and 9 to 10 are the SCAR18f/r and SCBC792f/r products amplified from LA317 (BB) (lanes 3 and 9) and Floradade (bb) (lanes 4 and 10). Lanes 5 and 6 are the SCAR18f/r SCAR products that were amplified and subsequently digested with RsaI for LA317 (BB) and Floradade (bb), respectively. Lanes 11 and 12 are the SCBC792f/r SCAR products that were amplified and subsequently digested with HinfI for LA317 (BB) and Floradade (bb) parents, respectively. Sizes of restriction enzyme digested fragments (Lanes 5–6 and 11–12) are indicated in Fig. 3 and 5.
