Fig. 1. Cloning of OPAR181100 and UBC792830 RAPD markers linked to the B locus by the T/A cloning technique. OPAR18 and UBC792 amplified polymorphic fragments were excised from agarose gels and cloned into pGEM-T Easy vector. The same-sized fragments (indicated by arrows) were recovered from EcoRI digested plasmid DNA. Lanes m and M are 1-kbp and 100-bp DNA ladders, respectively. Lanes 1 to 4 and 6 to 9 are individuals of EcoRI digested cloned plasmid DNA with OPAR181100 and UBC792830 inserts, respectively. Lanes 5 and 10 are RAPD fragments amplified with primers OPAR18 and UBC792, respectively. Lane 11 is a pGEM-T Easy vector.
