Fig. 8. Northern blot analysis using total RNA extracted from roots of the Mesilla and CUF 101 populations in December. Blots were probed with 32P-labeled cDNA for RootCAR1, a cold acclimation-responsive cDNA isolated from alfalfa roots whose expression is associated with alfalfa winter survival. See Fig. 2 for population definitions. The blot shown is typical of several obtained using different field replicates from either year. Insufficient root tissue of Mesilla-L2 was available for Northern analysis.
