Fig. 2. PCR amplification of the partial expression cassette NAP/FAE1/NOS using oligonucleotide primer NN-3 (5'-TTTCTTCGCCACTTGTCACTCC-3') which was designed according to the promoter region of the napin gene (position 948-969) and primer NN-4 (5'CGCGCTATATTTTGTTTTCTA-3') which was designed according to the nopaline-synthase 3' UTR sequence (position 1753–1773). The total size of the PCR product is approx. 2.0 kb (0.197 kb of napin promoter region + 1.608-kb FAE1 coding region + 0.204 kb of nopaline-synthase 3' UTR region); WS: Westar transgenic lines; WS-WT: Westar wild-type control line; C-: negative PCR control without DNA; H: Hero transgenic lines; H-WT: Hero wild-type control line; C+: positive PCR control—binary vector pNap:FAE1/NGKM.
