HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Free Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in ISI Web of Science Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via ISI Web of Science (3) Citing Articles via Google Scholar Google Scholar Articles by Steiner, J.J. Articles by Beuselinck, P.R. Search for Related Content PubMed Articles by Steiner, J.J. Articles by Beuselinck, P.R. Agricola Articles by Steiner, J.J. Articles by Beuselinck, P.R.

REGISTRATION OF GENETIC STOCKS

Registration of RG-BFT Photoperiod Insensitive and Rapid-Flowering Autogamous Birdsfoot Trefoil Genetic Stock

^a Research Agronomist, USDA-ARS, Natl. Forage Seed Prod. Res. Ctr., 3450 SW Campus Way, Corvallis, OR 97331
^b Research Geneticist, USDA-ARS, Plant Genetics Res. Unit, Univ. of Missouri, Columbia, MO 65211

Corresponding author (steinerj{at}ucs.orst.edu)

RG-BFT (Reg. no. GS-1, PI 613539) rapid reproductive regenerating and photoperiod insensitive birdsfoot trefoil (Lotus corniculatus L.) genetic stock was developed and released 25 May 2000 by the United States Department of Agriculture-Agricultural Research Service, in cooperation with the Oregon, Idaho, and Washington Agricultural Experiment Stations.

In November 1994, 2 g of AG-S4 (Steiner, 1993) seed was irradiated with 20 kR of gamma radiation, using a Gammacell 220 with a cobalt-60 source (Atomic Energy of Canada, Ottawa). A random 1200 plant population (AG-S4-IR) was grown from irradiated seed under greenhouse conditions of 16 h light day^-1 and 18°C. After flowering, self-pollination, and pod maturation, two pods per plant were collected from each plant and the seed threshed and cleaned. The harvested seed was designated IR-S₁. A second random 1200 plant population was grown from the IR-S₁ seed and handled as above. The reason for inducing mutations was to attempt to produce nonyellow-colored flower genotypes, but none were observed; however, a single clone (RG-S₁) that flowered more rapidly than all other clones was identified among the second grow-out population of IR-S₁ plants. Seed of RG-S₁ was germinated to produce 28 RG-S₂ plants. RG-BFT is a composite population of seed (RG-S₃) from the 28 plants.

RG-BFT was developed for inheritance studies of birdsfoot trefoil traits using Mendelian instead of population genetics. It is autogamous, does not require hand pollination to produce seed, and a greater number of reproductive cycles can be obtained in the same amount of time as typical flowering birdsfoot trefoil genotypes. RG-BFT flowers after receiving 550 accumulated heat units (10°C base temp) when grown under light periods ranging from 13 to 19 hr and using a light intensity averaging 410 mol m^-2 s^-1. When using similar light intensity and a 16 hr light period, AG-S4 and MU-81 (Beuselinck and McGraw, 1986) flower after 750 and 900 heat units, respectively. RG-BFT flowers after 1000 accumulated heat units when grown under 10-hr light period with a light intensity averaging 410 mol m^-2 s^-1. There are no other known birdsfoot trefoil genetic sources that flower under 10-hr photoperiods. Because RG-BFT is photoperiod insensitive, it also is a source of genes to modify photoperiod response in birdsfoot trefoil, and is suitable for basic research involving flowering. Though not grown under field conditions, it is anticipated that RG-BFT, like AG-S4 will not survive the winter in western Oregon.

RG-BFT crosses readily and bidirectionally with other birdsfoot trefoil genotypes, although when used as a female parent, it must be emasculated and has a lower percentage of pod set than when used as a male parent. RG-BFT has leaves that are of similar shape to those of AG-S4, has two to three flowers per umbel, and does not exhibit inbreeding depression. Based on random amplified polymorphic DNA, the band similarity percentages for RG-BFT with other birdsfoot trefoils include MU-81 (50%), MU-81-41 (AG-S4 progenitor) (43%), AG-S4 (8%), and autogamous USDA National Plant Germplasm System PI 260268 from Ethiopia (5%). The somatic chromosome number for RG-BFT is 2n = 4x = 24.

Limited amounts of RG-BFT seed will be provided upon written request to the corresponding author as supplies permit. Recipients are asked to recognize the source as a matter of open record when this genetic stock contributes to the development of a new germplasm or cultivar or is used for experimental purposes.

NOTES

Registration by CSSA.

Accepted for publication September 30, 2000.

REFERENCES

• Steiner, J.J. 1993. Registration of AG-S4 autogamous broad-leaf birdsfoot trefoil germplasm. Crop Sci. 33:1424–1425.[Free Full Text]
• Beuselinck, P.R., and R.L. McGraw. 1986. Registration of MU-81 birdsfoot trefoil germplasm. Crop Sci. 26:837–838.[Free Full Text]

This Article Full Text (PDF) Free Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in ISI Web of Science Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via ISI Web of Science (3) Citing Articles via Google Scholar Google Scholar Articles by Steiner, J.J. Articles by Beuselinck, P.R. Search for Related Content PubMed Articles by Steiner, J.J. Articles by Beuselinck, P.R. Agricola Articles by Steiner, J.J. Articles by Beuselinck, P.R.