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Fig. 3 Southern blot analysis for T0 transgenic plants and a nontransformed control plant. Gel blot lanes contained 25 µg of genomic DNA. Hybridization was carried out with a radio-labeled Cah gene. The arrow indicates the expected position of the 1661 bp Cah gene fragment. The migration position of the molecular weight markers are shown to the left of the autoradiogram and labeled with sizes in kilobases. The left three lanes contain undigested DNA and the next three lanes contain DNA digested with EcoRI. The lanes marked TA and TB represent genomic DNA from two independently transformed lines (plant lines 1650 and 1722, respectively). Nontransformed control DNA is designated as NT and lanes labeled RC contain 25 µg of genomic nontransformed DNA plus 0.592 pg of a 0.8-kb PstI digest sequence from the pCAM plasmid which should correspond to a single copy reconstruction
