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Fig. 2 Production of transgenic rice plants from embryogenic calli of mature seeds of Cocodrie via particle bombardment. (a) Somatic embryogenic calli induced from mature seeds in CI medium. (b) Proliferation of embryogenic calli in subculture CI medium. x 5. (c) Regeneration of embryogenic calli. x 10. (d) GUS gene expression in embryogenic calli 2 d after bombardment with plasmid pAHC25. The calli were stained with 5-bromo-4-chloro-3-indyl-ß-D-glucuronide (X-Gluc). (e) Growth of bombarded calli in the absence (left) vs. presence (right) of 4 mg L-1 bialaphos. (f) Plantlet regeneration (right plate) of transformed resistant calli compared to necrosis of the untransformed calli (left plate) in the PR-2 medium containing 4 mg L-1 bialaphos
