Fig. 2 Segregation of Kaybonnet M2-2045 in the M2 through M4 generations. Seeds produced by a given plant were individually crushed, and assayed for inorganic P. (A) M2 generation; A single M3 seed from each of 10 plants comprising a given M2 row were crushed, and tested. The rows shown are M2-2041 through M2-2048. (B) M3 generation, M3-2045-1 through M3-2045-9; 10 M4 seeds from each of nine M3 plants were tested. (C) M4 generation; a total of eight M5 seeds were tested for each of five sibling M4 plants representing the nine M3s in (B). The first four M5 tests for each M4 are shown. Standards: (1) 0.0 µg P; (2) 0.15 µg P; (3) 0.46 µg P; (4) 0.93 µg P; (5) 1.39 µg P. In this study, wild-type rice seeds typically have inorganic P levels ( = 0.15 mg P_i g^-1) that yield an assay result < Standard No. 2. Kaybonnet M2-2045 seeds have inorganic P levels that yield an assay result typically Standard No. 4, indicating these seeds contained 1.0 mg P_i g^-1

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