HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Free via Open Access OA Abstract OA Full Text Free Full Text (PDF) Free Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Johal, G. S. Articles by Weil, C. F. Search for Related Content PubMed Articles by Johal, G. S. Articles by Weil, C. F. Agricola Articles by Johal, G. S. Articles by Weil, C. F. Related Collections Germplasm Enhancement Functional Genomics Plant Genetic Resources

Mining and Harnessing Natural Variation: A Little MAGIC

^a Dep. of Botany and Plant Pathology, Purdue Univ., West Lafayette, IN 47907
^b USDA-ARS, Dep. of Plant Pathology, North Carolina State Univ., Raleigh, NC 27695
^c Dep. of Agronomy, Purdue Univ., West Lafayette, IN 47907

View larger version (3K): [in this window] [in a new window]   Figure 1. A hypothetical biosynthetic pathway controlled by five genes. The gene (e) controlling the last step is defective, causing little or no product being synthesized.

View larger version (13K): [in this window] [in a new window]   Figure 2. Second site suppression mutagenesis approach. M2 plants with arrows represent revertants in which the effect of the mutant phenotype has reversed. EMS, ethyl methane sulfonate; M1, the first generation following mutagenesis; M2, progeny of M1 individuals derived by self-pollination.

View larger version (14K): [in this window] [in a new window]   Figure 3. Mutant-assisted gene identification and characterization (MAGIC) using a recessive reporter mutation (P1). Arrows facing right point to F2 mutant segregants with a suppressed phenotype. The segregant with a left-facing arrow has an enhanced mutant phenotype.

View larger version (24K): [in this window] [in a new window]   Figure 4. Conducting mutant-assisted gene identification and characterization (MAGIC) with a dominant reporter mutation, used here as a heterozygote in an otherwise pure-breeding line (P1). P2 and P3 are two diverse lines. While P2 has a suppressible effect on the dwarfing phenotype of the mutant (A), P3 has an enhancing effect on this dwarfing mutant (B). In both (A) and (B), families are segregating 1:1 for mutant and wild-type sibs.