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Inheritance of Resistance to Alfalfa Mosaic Virus in Soybean PI 153282

^a Institute for Agronomy and Plant Breeding, Christian-Albrechts-Universität Kiel, Am Botanischen Garten 1-9, D-24118 Kiel, Germany
^b Dep. of Plant Pathology, Univ. of Wisconsin-Madison, Madison, WI 53706-1598
^c Dep. of Entomology, Univ. of Wisconsin-Madison, Madison, WI 53706-1598
^d Dep. of Crop Sciences, Univ. of Illinois, Urbana, IL 61801

View larger version (16K): [in this window] [in a new window]   Figure 1. Distribution of Alfalfa mosaic virus (AMV) visual severity (0 = asymptomatic to 3 = high level of symptoms) among 174 F4:7 lines derived from crossing the AMV susceptible cultivar S19-90 with the AMV resistance source PI 153282. The lines were tested for AMV resistance in a replicated greenhouse experiment. The bar patterns are according to the marker genotype of each F4:7 line for the marker Sat_228, which is the marker closest to the AMV resistance gene Rav1 based on the results of the quantitative trait locus (QTL) analysis (See Results and Fig. 2). F4:7 lines were homozygous for either parent allele (PI 153282 or S19-90) or still segregating at the Sat_228 locus.

View larger version (12K): [in this window] [in a new window]   Figure 2. Position, LOD curve, and additive effect of the major Alfalfa mosaic virus (AMV) resistance gene Rav1. The gene was mapped in a population of 174 F4:7 lines derived from crossing the AMV susceptible cultivar S19-90 with the AMV resistance source PI 153282. Rav1 was mapped to map position 25 on linkage group J within a support interval ( |——| ) ranging from map position 20 to 27. The additive effect of the resistance allele from PI 153282 is given on a visual scale of 0 = asymptomatic to 3 = high level of symptoms.

View larger version (29K): [in this window] [in a new window]   Figure 3. Distribution of Alfalfa mosaic virus (AMV) visual symptom (0 = asymptomatic to 3 = high level of symptoms) severity for F2 plants developed from crossing the AMV susceptible cultivar S19-90 with the AMV resistance source PI 153282. The population was tested in a greenhouse. The bar patterns are according to the genotype of each line for the marker Sat_228, which is the marker closest to the AMV resistance gene Rav1. F2 plants were homozygous for either parent allele (PI 153282 or S19-90) or heterozygous at the Sat_228 locus.

View larger version (18K): [in this window] [in a new window]   Figure 4. Distribution of optical density (OD) readings (405 nm) for an indirect enzyme-linked immunosorbent assay (ELISA) of alfalfa mosaic virus (AMV)-inoculated F2 plants developed from crossing the AMV susceptible cultivar S19-90 with the AMV resistance source PI 153282. The population was tested in the greenhouse. The bar patterns are according to the genotype of each line for the marker Sat_228, which is the marker closest to the AMV resistance gene Rav1. F2 plants were either homozygous for either parent allele (PI 153282 or S19-90) or heterozygous at the Sat_228 locus.

View larger version (13K): [in this window] [in a new window]   Figure 5. Position and LOD curve for the major Alfalfa mosaic virus (AMV) resistance gene Rav1. The gene was mapped on linkage group J in a population of 220 F2 plants developed from crossing the AMV susceptible cultivar S19-90 with the AMV resistance source PI 153282. Rav1 mapped on map position 24 (LOD = 6.7) with an upper end support boundary (——| ) at map position 28 based on visual symptom severity. Based on indirect enzyme-linked immunosorbent assay (ELISA) data, Rav1 mapped to position 25 (LOD = 24.7) with an upper end support boundary at map position 28.

View larger version (11K): [in this window] [in a new window]   Figure 6. Position and LOD curve for the major gene Alfalfa mosaic virus (AMV) resistance gene Rav1. The gene was mapped on linkage group J using selected F2:3 lines from a population developed from crossing the AMV susceptible cultivar S19-90 with the AMV resistance source PI 153282. The selected lines include 40 derived from AMV susceptible F2 plants and 40 derived from AMV resistant F2 plants. Rav1 mapped on map position 30 (LOD = 8.0) with an support interval ranging from map position 27 to 34 ( |——| ) based on visual symptoms. Based on indirect enzyme-linked immunosorbent assay (ELISA) Rav1 mapped on map position 27 (LOD = 4.6) with an upper end support boundary (——| ) on map position 32.