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Micropropagation of Field-Grown Perennial Teosinte from Node Culture

^a Dep. of Plant Sciences, Univ. of Tennessee, Rm. 252 Ellington, 2431 Joe Johnson Dr., Knoxville, TN 37996-4561
^b Univ. of Tennessee, Center for Biomarker Analysis, 10515 Research Dr., Suite 300, Knoxville, TN 37932-2575
^c present address: Institute of Experimental Botany, Academy of Sciences of the Czech Republic, Sokolovská 6, 77200 Olomouc, Czech Republic
^d contributed equally to this work

View larger version (31K): [in this window] [in a new window]   Figure 1. Schematic diagram of teosinte explant preparation.

View larger version (227K): [in this window] [in a new window]   Figure 2. Longitudinal section of Zea perennis showing one axillary bud per node. MB = main branch; AX = axillary branch. Scale bar 20 µm.

View larger version (84K): [in this window] [in a new window]   Figure 3. Cross-section of a Zea diploperennis node showing a single leaf trace (LT). VB = vascular bundles; EP = epidermis. Scale bar 50 µm.

View larger version (64K): [in this window] [in a new window]   Figure 4. Composite collection of cross-sections at a node showing scattered vascular bundles of Zea diploperennis using widefield deconvolution microscopy. Scale bar 100 µm.

View larger version (89K): [in this window] [in a new window]   Figure 5. The differences in the number and vigor of multiple shoots between (A) Zea diploperennis and (B) Z. perennis grown on Murashige and Skoog's medium with 5 µM 6-benzyl amino purine and 3% w/v sucrose.