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Mapping Genes Encoding Microsomal -6 Desaturase Enzymes and Their Cosegregation with QTL Affecting Oleate Content in Soybean

^a Dep. of Crop Science, North Carolina State Univ., Campus Box 7620, Raleigh, NC 27695-7620
^b USDA-ARS, Soybean and Nitrogen Fixation Research Unit, 3127 Ligon St., Raleigh, NC 27607

View larger version (72K): [in this window] [in a new window]   Figure 1. Alignment of the 395-bp fragment amplified for FAD2-1B cleaved amplified polymorphic sequence analysis in N98-4445A, N97-3363-3, PI423893, and ‘Brim’. The primers FAD2-1B_c_F and FAD2-1B_b_R designed for amplification are italicized. The restriction sites for HpyCH4III are underlined and the three single nucleotide polymorphisms (SNPs) are highlighted. The substitution from thymine to cytosine in one of the SNPs generates an additional restriction site for HpyCH4III endonuclease in PI423893.

View larger version (24K): [in this window] [in a new window]   Figure 2. Genetic map of Linkage Groups I, O, and L for the FAF population (N97-3363-3 x PI423893) constructed with JoinMap 3.0. The estimated locations of FAD2-1A, FAD2-2A, and FAD2-2B are presented. It should be noted that mapping of FAD2-2B on Linkage Group L in the FAF population is not in agreement with linkage analysis in the FAS population. The FAD2-2B isoform probably maps within the interval of the simple sequence repeat markers sat_340 and satt006 on Linkage Group L, as indicated by the linkage map of FAS population (N98-4445A x PI423893). The FAD2-2A isoform also colocalizes with FAD2-2B on Linkage Group L. Genetic distances were estimated with Kosambi's mapping function. Linkage groups were drawn on an equal scale.