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Molecular Marker-Assisted Backcrossing of Anthracnose Resistance into Andean Climbing Beans (Phaseolus vulgaris L.)

^a Universidad Nacional de Colombia, Facultad de Agronomía, Ciudad Universitaria, Bogotá D.C.- Colombia, Av. Carrera 30 N° 45-03
^b International Center for Tropical Agriculture (CIAT), Recta Cali-Palmira, Km. 17. A.A.6713, Cali-Colombia

View larger version (72K): [in this window] [in a new window]   Figure 1. PCR amplification of the proteinase K (PK) extraction DNA (panel A) and alkaline extraction (AE) method DNA (panel B) for the SCAR markers SAS13 and SAB3 in the anthracnose differential genotypes from Pastor-Corrales (1991): MDRK (lanes 1 in both panels and both markers), ‘Perry Marrow’ (lanes 2), ‘Kaboon’ (lanes 3), ‘Michelite’ (lanes 4), G2333 (lanes 5), AB136 (lanes 6), Cornell 47–292 (lanes 7), Mexico 222 (lanes 8), PI 207262 (lanes 9), ‘Widusa’ (lanes 10), TO (lanes 11), TU (lanes 12). Lane 13 represents control reaction of proteinase K miniprep DNA for G2333 in panel B with molecular weight ladder (-HindIII) indicated with ‘M’ in both panels.

View larger version (76K): [in this window] [in a new window]   Figure 2. SAB3 marker amplification in a subset of BC1F1 plants from the marker-assisted backcross-breeding program using either DNA from a) the PK method or b) the AE method. Lanes 1– 15 represent individual plants from the cross Pesca x (Pesca x G2333). Molecular weight ladder (M) indicated in left hand lane. Positive (R) and negative (S) control genotypes, G2333 and Pesca indicated in right hand lanes. Arrows indicate false negatives in the PCR amplification of the PK method DNA.