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Pyramiding of Soybean Mosaic Virus Resistance Genes by Marker-Assisted Selection

^a Dep. of Crop & Soil Environmental Sciences, Virginia Tech, Blacksburg, VA 20461
^b BioEvaluation Center, Korea Research Institute of Bioscience and Biotechnology, Chungbuk 363-883, Republic of Korea
^c Phillip Morris USA, Research Center, Richmond, VA 23261
^d Dep. of Plant Pathology, Physiology & Weed Science, Virginia Tech, Blacksburg, VA 24061

View larger version (35K): [in this window] [in a new window]   Figure 1. Schematic diagram showing marker-assisted selection for pyramiding the three major genes (Rsv1, Rsv3, and Rsv4) for soybean mosaic virus (SMV) resistance.

View larger version (51K): [in this window] [in a new window]   Figure 2. Polyacrylamide gel showing the separation of the alleles of polymerase chain reaction–based molecular markers. The microsatellite marker genotyping of three F1 plants obtained by the crosses between ‘Essex’ isolines containing two soybean mosaic virus resistance genes is shown. The expected genotypes of F1 plants and their parental plants are listed in the inset.

View larger version (84K): [in this window] [in a new window]   Figure 3. (A) Essex Rsv1 donor source, (B) PI96983, and (C) Rsv1 Essex isoline V00-7266 inoculated with SMV-G7 14 d postinoculation (dpi). Reaction of (D) Essex, (E) L29, parental source of Rsv3, and (F) isoline V239 inoculated with SMV-G2 14 dpi. (G) Rsv3Rsv4 pyramided Essex isoline V01-7341-1 characterized late susceptible reaction inoculated with SMV-G1 35 dpi. (H) Immunoprint of the leaf pictured in Fig. 3G showing distribution exhibiting nonrandom distribution of virus indicated by dark areas on the leaf blot.