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Marker-Assisted Selection in Plant Breeding: From Publications to Practice

Genetic Resources and Enhancement Unit, International Maize and Wheat Improvement Center (CIMMYT), Apdo, Postal 6-641, 0660 Mexico, D.F., Mexico

View larger version (16K): [in this window] [in a new window]   Figure 1. The numbers of articles with the terms quantitative trait locus or quantitative trait loci (QTL) and marker-assisted selection (MAS) by years (1984–2005) from Google Scholar (4 Aug. 2007).

View larger version (38K): [in this window] [in a new window]   Figure 2. Flowchart for large-scale selective genotyping and genetic mapping, including selection of phenotypic extremes from large-size segregating populations, phenotype confirmation, DNA extraction, genotyping, and marker–trait association analysis. (A) A procedure for most target traits which can be scored phenotypically for all individuals or fixed lines, and then high- and low-phenotypic extremes are selected for further analysis. (B) A procedure particularly suitable for abiotic and biotic stress tolerance where only the phenotypic extreme for tolerance is available under a target environment and comparison is made between the extreme and the phenotypic control that is randomly selected from the individuals or fixed lines under a normal environment.