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A PCR-based Marker for the Rsv1 Locus Conferring Resistance to Soybean Mosaic Virus

Dep. of Crop, Soil, and Environmental Sciences, Univ. of Arkansas, Fayetteville, AR 72701

View larger version (57K): [in this window] [in a new window]   Figure 1. DNA fragments amplified from the primer pair Rsv1-f/r (Lanes 1–8) on 1.3% agarose gel. 1, Essex (rsv); 2, PI 96983 (Rsv1); 3, Marshall (Rsv1-m); 4, York (Rsv1-y); 5, Raiden (Rsv1-r); 6, Kwanggyo (Rsv1-k); 7, Ogden (Rsv1-t); and 8, Suweon 97 (Rsv1-h). Lane M is a 100-bp molecular-weight marker.

View larger version (25K): [in this window] [in a new window]   Figure 2. Amplification patterns of the marker with the primer pair Rsv1-f/r in 33 plants of the F2 population derived from J05 x Essex on 1.3% agarose gel. R, resistant plant; S, susceptible plant.

View larger version (48K): [in this window] [in a new window]   Figure 3. Amplification patterns of the DNA fragments with the simple sequence repeat (SSR) primer Satt 114 in 34 plants of the F2 population derived from J05 x Essex and two parents on 6% nondenaturing polyacrylamide gel. P1, J05; P2, Essex; R, resistant F2 plant; S, susceptible F2 plant. Lane M is a 100-bp molecular-weight marker.

View larger version (13K): [in this window] [in a new window]   Figure 4. A genetic map around the 3gG2 gene at the Rsv1 locus region between Sat_297 at 59.60 cM and Sct_033 at 74.13 cM of the molecular linkage group (MLG) F. The map was constructed using simple sequence repeat (SSR) marker Satt 114 and specific primer pair Rsv1-f/r for the 3gG2 gene in the J05 x Essex population combined with eight existing SSR makers as reference from the soybean molecular linkage map by Cregan (2003).